ANALYSIS OF ECO ENZYME CHARACTERISTICS WITH VARIATION OF “TAPE” YEAST CONCENTRATIONS

Miftahul Karimah; Cucuk Evi Lusiani

doi:10.33795/distilat.v10i1.4195

Authors

Miftahul Karimah Department of Chemical Engineering, Politeknik Negeri Malang, Soekarno Hatta Street No.9, Malang 65141, Indonesia
Cucuk Evi Lusiani Department of Chemical Engineering, Politeknik Negeri Malang, Soekarno Hatta Street No.9, Malang 65141, Indonesia

DOI:

https://doi.org/10.33795/distilat.v10i1.4195

Keywords:

acetic acid, eco enzyme, pH, “tape” yeast, TDS

Abstract

Eco enzyme is a multipurpose liquid which produced from organic waste. The liquid made with the ratio of raw materials in the form of brown sugar: organic waste: water is 1:3:10 which is processed using a fermentation process for 90 days. One method to shorten the fermentation time is adding yeast as catalyst throughout the fermentation process. This research aims to analyze the characteristics of the eco enzyme with variations in “tape” yeast concentrations. Raw material used in this research included organic waste (orange and melon peels with a ratio of 1:1), brown sugar, water, and “tape” yeast (yeast concentrations of 1, 2, 3, 4 and 5%w/v) through fermentation process for ten days. The eco enzyme products were tested to determine the pH value, TDS value, acetic acid content, aroma, color and protease enzyme activity. The analysis results showed that the pH value of eco enzyme products was in the range of 3.9 to 4.1, TDS (Total Dissolved Solids) value of 1339 to 1405 ppm, acetic acid content of 0.81 to 1.08%w/w, had bright yellow and deep yellow color with a fermented aroma. The selected eco enzyme product in this research was on eco enzyme with yeast concentration of 3 %w/v which had pH value of 3.9 TDS value 1403 ppm, acetic acid content of 0.81%w/w, and the color was bright yellow with fermented aroma. However, there was no clear zone around the colony in the test indicating that there was no protease enzyme activity.

References

U. Septiani, Najmi, and R. Oktavia, “Eco Enzyme : Pengolahan Sampah Rumah Tangga Menjadi Produk Serbaguna di Yayasan Khazanah Kebajikan,” J. Univ. Muhamadiyah Jakarta, vol. 02, no. 1, hal. 1–7, 2021.

A. A. Kadir, N. W. Azhari, and S. N. Jamaludin, “An Overview of Organic Waste in Composting,” MATEC Web Conf., vol. 47, hal. 0–5, 2016.

Y. Dhokhikah and Y. Trihadiningrum, “Solid Waste Management in Asian Developing Countries: Challenges and Opportunities,” J. Appl. Environ. Biol. Sci. J. Appl. Environ. Biol. Sci., vol. 2, no. 7, hal. 329–335, 2012.

Y. Hasanah, L. Mawarni, and H. Hanum, “Eco Enzyme and Its Benefits for Organic Rice Production and Disinfectant,” J. Saintech Transf., vol. 3, no. 2, hal. 119–128, 2020.

S. Dhiman, “Eco-Enzyme-A Perfect House-Hold Organic Cleanser,” Int. J. Eng. Technol. Manag. Appl. Sci., vol. 5, no. 11, hal. 19–23, 2017.

V. Lapsia and M. N. Cherekar, “Production, Extraction and Uses of Eco Enzyme Using Citrus Fruit Waste: Wealth from Waste,” Asian Jr. Microbiol. Biotech. Env. Sc, vol. 22, no. 2, hal. 346–351, 2020.

O. Galintin, N. Rasit, and S. Hamzah, “Production and Characterization of Eco Enzyme Produced from Fruit and Vegetable Wastes and Its Influence on The Aquaculture Sludge,” Biointerface Res. Appl. Chem., vol. 11, no. 3, hal. 10205–10214, 2021.

M. Rijal, “Application of Eco-enzymes from Nutmeg, Clove, and Eucalyptus Plant Waste in Inhibiting the Growth of E. coli and S. aureus In Vitro,” BIOSEL (Biology Sci. Educ. J. Penelit. Sci. dan Pendidik., vol. 11, no. 1, hal. 31–44, 2022.

H. K. Oktaviani and C. E. Lusiani, “Pengaruh Waktu Fermentasi Terhadap Virgin Coconut Oil (Vco) Dari Kelapa Daerah Probolinggo Menggunakan Ragi Tempe 2% B/V,” Distilat J. Teknol. Separasi, vol. 7, no. 2, hal. 282–287, 2021.

A. M. Buswell and W. D. Hatfield, Anaerobic Fermentations, no. 32. Urbana, Illinois, 1936.

D. Suprayogi, R. Asra, and R. Mahdalia, “Analisis Produk Eco Enzyme dari Kulit Buah Nanas (Ananas comosus L.) dan Jeruk Berastagi (Citrus X sinensis L.),” J. Redoks, vol. 7, no. 1, hal. 20–1, 2022.

N. Rasit, L. H. Fern, and A. W. A. K. Ghani, “Production and Characterization of Eco Enzyme Produced From Tomato and Orange Wastes and Its Influence On The Aquaculture Sludge,” Int. J. Civ. Eng. Technol., vol. 10, no. 03, hal. 967–980, 2019.

D. Imelda, A. B. Alif, and B. D. Satriawan, “Pembuatan Produk Multipurpose Cleaner dengan Pemanfaatan Eco Enzyme dari Limbah Kulit Buah sebagai Bahan Aktif Natural Antimikroba,” Universitas Jayabaya, Jakarta, 2021.

N. Rochyani, R. L. Utpalasari, and I. Dahliana, “Analisis Hasil Konversi Eco Enzyme Menggunakan Nenas (Ananas comosus ) dan Pepaya (Carica papaya L.),” J. Redoks, vol. 5, no. 2, hal. 135, 2020.

A. Novianti and I. N. Muliarta, “Eco-Enzym Based on Household Organic Waste as Multi-Purpose Liquid,” Agriwar J., vol. 1, no. 1, hal. 12–17, 2021.

M. R. Rahayu, I. N. Muliarta, and Y. P. Situmeang, “Acceleration of Production Natural Disinfectants from the Combination of Eco-Enzyme Domestic Organic Waste and Frangipani Flowers (Plumeria alba),” SEAS (Sustainable Environ. Agric. Sci., vol. 5, no. 1, hal. 15–21, 2021.

M. M. Maharani, M. Bakrie, and Nurlela, “Pengaruh Jenis Ragi, Massa Ragi dan Waktu Fermentasi pada Pembuatan Bioetanol dari Limbah Biji Durian,” J. Redoks, vol. 6, no. 1, hal. 57, 2021.

A. Islami, “Identifikasi Kadar Asam Asetat pada Ecoenzyme dari Bahan Organik Kulit Jeruk dengan Metode Titrasi Asam Basa,” Universitas Negeri Padang, 2022.

R. Poompanvong, Modul Pembuatan Eco-Enzyme. 2020.

A. Etienne, M. Génard, P. Lobit, D. Mbeguié-A-Mbéguié, and C. Bugaud, “What Controls Fleshy Fruit Acidity? A Review of Malate and Citrate Accumulation in Fruit Cells,” J. Exp. Bot., vol. 64, no. 6, hal. 1451–1469, 2013.

S. Sambaraju and V. Sree Lakshmi, “Eco-friendly Treatment of Dairy Wastewater Using Garbage Enzyme,” Mater. Today Proc., vol. 33, hal. 650–653, 2020.

N. Jamil, R. Jabeen, M. Khan Musarat Riaz, T. Naeem, A. Khan, N. U. Sabah, S. A. Ghori, U. Jabeen, Z. A. Bazai, A. Mushtaq, S. Rizwan, and S. Fahmid, “Quantitative Assessment of Juice Content, Citric Acid and Sugar Content in Oranges, Sweet Lime, Lemon and Grapes Available in Fresh Fruit Market of Quetta City,” Int. J. Basic Appl. Sci., vol. 15, no. 1, 2020.

B. A. Kartika, P. Hastuti, and W. Supartono, Pedoman Uji Inderawi Bahan Pangan. 1988.

A. Yuliono, M. S. J Sofiana, I. Safitri, Warsidah, A. Antasari, Kushadiwijayanto, and S. Helena, “Peningkatan Kesehatan Masyarakat Teluk Batang secara Mandiri melalui pembuatan Handsanitizer dan Desinfektan berbasis Eco-Enzyme dari Limbah Sayuran dan Buah,” J. Community Engagem. Heal., vol. 4, no. 2, hal. 371–377, 2021.

S. P. Dewi, S. Devi, and S. Ambarwati, “Pembuatan dan Uji Organoleptik Eco-enzyme dari Kulit Buah Jeruk,” hal. 649–657, 2021.

M. Rijal, Surati, I. Amir, A. Abdollah, A. B. Lessy, A. S. Ytatroman, N. Tanama, Eco-Enzyme dari Limbah Tanaman Maluku, 1st ed. Ambon: LP2M IAIN Ambon, 2021.