Application of Sequencing Batch Biofilm Reactor (SBBR) Using Microalgae Chorella sp. to Removal Nutrient in Grey Water

Authors

  • Shinta Elystia Department of Environmental Engineering, Faculty of Engineering, Universitas Riau, Kampus Bina Widya Jl. HR. Soebrantas Km 12,5 Simpang Baru, Pekanbaru 28293, Indonesia
  • Rika Kristin Department of Environmental Engineering, Faculty of Engineering, Universitas Riau, Kampus Bina Widya Jl. HR. Soebrantas Km 12,5 Simpang Baru, Pekanbaru 28293, Indonesia
  • David Andrio Department of Environmental Engineering, Faculty of Engineering, Universitas Riau, Kampus Bina Widya Jl. HR. Soebrantas Km 12,5 Simpang Baru, Pekanbaru 28293, Indonesia

DOI:

https://doi.org/10.33795/jtkl.v7i1.338

Keywords:

Chlorella sp., grey water, sequencing batch biofilm reactor, stabilization time

Abstract

Grey water contains organic matter that is directly disposed to the environment without any treatment previously, will cause pollution and impacting life in the water. Treatment that can be done is using microorganisms. One of its kind is the microalgae Chlorella sp. which utilizes organic matter as a source of nutrients for its growth. In this study, the Kaldness 1 (K1) bio carrier was added as a medium for attaching microorganisms using the Sequencing Batch Biofilm Reactor (SBBR) process. The research objectives were (1) to know the maximum number of Chlorella sp. both attached and suspended in the Sequencing Batch Biofilm Reactor (SBBR), (2) to obtain the best cycle time and stabilization time in the removal of COD, Ammonia, and MLSS in grey water. The research was conducted by varying the stabilization time (1.5; 2 and 2.5 hours) in each cycle for four cycles with a constant variation of charging time 30 minutes, reaction 120 minutes, 45 minutes, separation 45 minutes, and carried out with four cycles, stirring speed at 60 rpm, the concentration of algae suspension in SBBR was 25% and the volume of Kaldness K1 medium was 20%. The results showed the number of microalgae cells Chlorella sp. was suspended and attached to 1.85 x 106 and 1.46 x 106 cells/ml. The best removal of COD, ammonia, and MLSS was found in the stabilization time variation of 1.5 hours in 4 cycles with a removal efficiency of 84% and 76%, respectively, and an increase in the concentration of suspended and attached MLSS by 4780 mg/l and 4720 mg/l. It can be concluded that the faster stabilization time, the more removal efficient will be.

References

S. S. Chan, K. S. Khoo, K. W. Chew, T. C. Ling, P. L. Show, Recent advances biodegradation and biosorption of organic compounds from wastewater: Microalgae-bacteria consortium - A review, Bioresour. Technol., vol. 344, pp. 126159, 2022.

P. D. Patil, V. P. Bhange, S. S. Shende, P. S. Ghorpade, Greywater characterization of an Indian household and potential treatment for reuse, Water-Energy Nexus, vol. 5, pp. 1–7, 2022.

I. N. Shaikh, M. M. Ahammed, M. P. S. Krishnan, Graywater treatment and reuse, in Sustainable Water and Wastewater Processing, Amsterdam: Elsevier, pp. 19–54, 2019.

D. K. Kanaujiya, T. Paul, A. Sinharoy, K. Pakshirajan, Biological Treatment Processes for the Removal of Organic Micropollutants from Wastewater: a Review, Curr. Pollut. Reports, vol. 5, no. 3, pp. 112–128, 2019.

T. Prartono, M. Kawaroe, D. W. Sari, D. Augustine, Fatty Acid Content of Indonesian Aquatic Microalgae, HAYATI J. Biosci., vol. 17, no. 4, pp. 196–200, 2010.

A. H. Jagaba, S. R. M. Kutty, A. Noor, A. H. Birniwa, A. C. Affam, I. M. Lawal, M. U. Kankia, A. U. Kilaco, A systematic literature review of biocarriers: Central elements for biofilm formation, organic and nutrients removal in sequencing batch biofilm reactor, J. Water Process Eng., vol. 42, pp. 102178, 2021.

E. Ashrafi, E. Allahyari, E. Torresi, H. R. Andersen, Effect of slow biodegradable substrate addition on biofilm structure and reactor performance in two MBBRs filled with different support media, Environ. Technol., vol. 41, no. 21, pp. 2750–2759, 2020.

F. Jaramillo, M. Orchard, C. Muñoz, M. Zamorano, C. Antileo, Advanced strategies to improve nitrification process in sequencing batch reactors - A review, J. Environ. Manage., vol. 218, pp. 154–164, 2018.

Q. He, L. Chen, S. Zhang, R. Chen, H. Wang, Hydrodynamic shear force shaped the microbial community and function in the aerobic granular sequencing batch reactors for low carbon to nitrogen (C/N) municipal wastewater treatment, Bioresour. Technol., vol. 271, pp. 48–58, 2019.

B. M. Mareai, M. Fayed, S. A. Aly, W. I. Elbarki, Performance comparison of phenol removal in pharmaceutical wastewater by activated sludge and extended aeration augmented with activated carbon, Alexandria Eng. J., vol. 59, no. 6, pp. 5187–5196, 2020.

Y. J. Chan, M. F. Chong, C. L. Law, Biological treatment of anaerobically digested palm oil mill effluent (POME) using a Lab-Scale Sequencing Batch Reactor (SBR), J. Environ. Manage., vol. 91, no. 8, pp. 1738–1746, 2010.

J. Harahap, T. Gunawan, S. Suprayogi, M. Widyastuti, A review: Domestic wastewater management system in Indonesia, IOP Conf. Ser. Earth Environ. Sci., vol. 739, no. 1, pp. 12031, 2021.

S. Elystia, M. D. Amelia, S. R. Muria, Pengaruh variasi laju alir gas CO2 terhadap penyisihan COD dan penyerapan CO2 oleh Chlorella sp. menggunakan flat-photobioreactor pada POME, J. Teknol. Pertan. Andalas, vol. 24, no. 1, pp. 44–53, 2016.

J. Hussain, X. Wang, L. Sousa, R. Ali, B. E. Rittmann, W. Liao, Using non-metric multi-dimensional scaling analysis and multi-objective optimization to evaluate green algae for production of proteins, carbohydrates, lipids, and simultaneously fix carbon dioxide, Biomass and Bioenergy, vol. 141, pp. 105711, 2020.

T. Lines, P. Orr, J. Beardall, Elevated CO2 has Differential Effects on Five Species of Microalgae from a Subtropical Freshwater Lake: Possible Implications for Phytoplankton Species Composition, J. Phycol., vol. 57, no. 1, pp. 324–334, 2021.

Y. Torres-Tiji, F. J. Fields, S. P. Mayfield, Microalgae as a future food source, Biotechnol. Adv., vol. 41, pp. 107536, 2020.

S. Elystia, S. R. Muria, S. I. P. Pertiwi, Pemanfaatan Mikroalga Chlorella sp untuk Produksi Lipid dalam Media Limbah Cair Hotel dengan Variasi Rasio C:N dan Panjang Gelombang Cahaya, J. Sains & Teknologi Lingkung., vol. 11, no. 1, pp. 25–43, 2019.

L. R. de Assis, M. L. Calijuri, P. P. Assemany, E. C. Berg, L. V. Febroni, T. A. Bartolomeu, Evaluation of the performance of different materials to support the attached growth of algal biomass, Algal Res., vol. 39, pp. 101440, 2019.

R. F. Darmayanti, M. Muharja, T. Zhao, M. Gao, Y. Tashiro, K. Sakai, K. Sonomoto, Techno-Economic Analysis of Extractive Butanol Fermentation by Immobilized Cells with Large Extractant Volume, J. Tek. Kim. dan Lingkung., vol. 6, no. 2, pp. 99–111, 2022.

D. Iriani, B. Hasan, H. S. Putra, T. M. Ghazali, Optimization of Culture Conditions on Growth of Chlorella sp. Newly Isolated From Bagansiapiapi Waters Indonesia, IOP Conf. Ser. Earth Environ. Sci., vol. 934, no. 1, pp. 12097, 2021.

S. Mustafa, H. N. Bhatti, M. Maqbool, M. Iqbal, Microalgae biosorption, bioaccumulation and biodegradation efficiency for the remediation of wastewater and carbon dioxide mitigation: Prospects, challenges and opportunities, J. Water Process Eng., vol. 41, pp. 102009, 2021.

D. Moentamaria, M. Muharja, T. Widjaja, A. Widjaja, A performance study of home-made co-immobilized lipase from mucor miehei in polyurethane foam on the hydrolysis of coconut oil to fatty acid, Bull. Chem. React. Eng. Catal., vol. 14, no. 2, pp. 391–403, 2019.

D. Moentamaria, M. Muharja, T. Widjaja, A. Widjaja, Thermal stability and reusability of home-made co-immobilized lipase from Mucor miehei in polyurethane foam for the production of bio-flavor, IOP Conf. Ser. Mater. Sci. Eng., vol. 543, no. 1, pp. 012025, 2019.

S. Elystia, Pengolahan kandungan COD limbah cair pabrik kelapa sawit oleh Typha latifolia dengan metode fitoremediasi, J. Dampak, vol. 11, no. 2, pp. 88–95, 2014.

Y. Zalfiatri, F. Restuhadi, A. Pramana, F. Yuliandri, Decreasing Levels of BOD, COD and Oil in Palm Oil Mill Effluent (POME) Using Scale Up Experiment of Symbiosis Mutualism Technology Between Microalgae Chorella Sp and Agrobost, J. Appl. Agric. Sci. Technol., vol. 4, no. 2, pp. 170–180, 2020.

D. C. Rajput, A. K. Khambete, Performance of Sequencing Batch Biofilm Reactor on Low Filling Ratio to Treat Sewage, Int. J. Innov. Res. Sci. Technol., vol. 1, no. 10, pp. 269–273, 2015.

A. S. Setiyawan, A. Nur, M. Fauzi, K. Oginawati, P. Soewondo, Effects of Different Polymeric Materials on the Bacterial Attachment and Biofilm Formation in Anoxic Fixed-Bed Biofilm Reactors, Water, Air, Soil Pollut., vol. 234, no. 3, pp. 147, 2023.

G. Mujtaba, M. Rizwan, K. Lee, Removal of nutrients and COD from wastewater using symbiotic co-culture of bacterium Pseudomonas putida and immobilized microalga Chlorella vulgaris, J. Ind. Eng. Chem., vol. 49, pp. 145–151, 2017.

G. Salbitani, S. Carfagna, Ammonium Utilization in Microalgae: A Sustainable Method for Wastewater Treatment, Sustainability, vol. 13, no. 2, pp. 956, 2021.

A. A. L. Zinatizadeh, E. Ghaytooli, Simultaneous nitrogen and carbon removal from wastewater at different operating conditions in a Moving Bed Biofilm Reactor (MBBR): Process modeling and optimization, J. Taiwan Inst. Chem. Eng., vol. 53, pp. 98–111, 2015.

H. Jia, Q. Yuan, Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia, Cogent Environ. Sci., vol. 2, no. 1, pp. 1275089, 2016.

R. Septifani, S. Suhartini, I. J. Perdana, Cleaner production analysis of tofu small scale enterprise, IOP Conf. Ser. Earth Environ. Sci., vol. 733, no. 1, pp. 12055, 2021.

A. S. Afifah, I. W. K. Suryawan, A. Sarwono, Microalgae production using photo-bioreactor with intermittent aeration for municipal wastewater substrate and nutrient removal, Commun. Sci. Technol., vol. 5, no. 2, pp. 107–111, 2020.

K. Xiao, S. Liang, X. Wang, C. Chen, X. Huang, Current state and challenges of full-scale membrane bioreactor applications: A critical review, Bioresour. Technol., vol. 271, pp. 473–481, 2019.

S. Elystia, H. S. Edward, A. E. Putri, Removal of Chromium (VI) and Chromium (III) by using Chlorella sp Immobilized at Electroplating Wastewater, IOP Conf. Ser. Earth Environ. Sci., vol. 515, no. 1, pp. 12078, 2020.

Downloads

Published

2023-04-30

Issue

Section

Articles