Synthesis of Methyl Ester from Microalgae Chlorella sp. TAD Using the In-Situ Transesterification Method

Ivon Telussa; Eirene G. Fransina; Kalsum Kalauw

doi:10.33795/jtkl.v8i1.3197

Authors

Ivon Telussa Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Pattimura, Jl. Ir M Putuhena, Kampus Unpatti Poka Ambon 97233, Indonesia
Eirene G. Fransina Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Pattimura, Jl. Ir M Putuhena, Kampus Unpatti Poka Ambon 97233, Indonesia
Kalsum Kalauw Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Pattimura, Jl. Ir M Putuhena, Kampus Unpatti Poka Ambon 97233, Indonesia

DOI:

https://doi.org/10.33795/jtkl.v8i1.3197

Keywords:

Chlorella sp. TAD, in-situ transesterification, methyl ester, microalgae, synthesis

Abstract

Synthesis of methyl ester from Chlorella sp. TAD microalgae was carried out using the in-situ transesterification method. This study aims to determine the methyl ester composition of Chlorella sp. TAD microalgae using the in-situ transesterification method. The in-situ transesterification method is a modified method that allows extraction and transesterification into methyl ester products in one process simultaneously. The in-situ transesterification process lasted 8 hours, followed by a distillation process to remove the n-hexane content and an oven for 2 hours to evaporate the remaining water. The results of the analysis using GC-MS to determine the chemical content of the methyl ester compound from Chlorella sp. TAD, showed the methyl ester composition of 7,10-hexadecanoic methyl ester, 8,11,14-docosatrienoic methyl ester, 9-hexadecanoic methyl ester, hexadecanoic methyl ester, heptadecanoic methyl ester, 10-octadecenoic methyl ester, octadecenoic methyl ester, 9,12-octadecadienoic methyl ester, 10-octadecenoic methyl ester, 2-hexyl cyclopropaneoctanoic methyl ester, eicosanoic methyl ester, 10-heptadecen-8-ynoic acid methyl ester, nonahexacontanoic methyl ester, and tetracosanoic methyl ester.

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