Optimization of Operating Conditions in Lignin Isolation Process of Rice Straw Using Box-Behnken Design Methodology

Lintang Alivia Anggerta; Listiyana Candra Dewi

doi:10.33795/jtkl.v9i1.6886

Authors

Lintang Alivia Anggerta Department of Chemical Engineering, Politeknik Negeri Malang, Jl. Soekarno Hatta No. 9, Malang 65141, Indonesia
Listiyana Candra Dewi Department of Chemical Engineering, Politeknik Negeri Malang, Jl. Soekarno Hatta No. 9, Malang 65141, Indonesia

DOI:

https://doi.org/10.33795/jtkl.v9i1.6886

Keywords:

Box-Behnken methodology, isolation, lignin, optimization, sunscreen, ultraviolet radiation

Abstract

Exposure to sunlight causes ultraviolet (UV) radiation, which can damage the body cells. Those negative effects can be prevented by using sunscreen, which contains lignin. Lignin has been proven to absorb or reflect UV radiation effectively. Rice straw was being used as a non-synthetic active ingredient in sunscreen production. This research shows that during the operating conditions of the isolation process, several variables affect yield production, such as the mass of rice straw, temperature and time of isolation. Therefore, this research was carried out to optimize the lignin isolation process using the Box-Behnken Design (BBD) methodology. Rice straw dregs that have undergone sample preparation were subsequently isolated through hydrolysis using 5% (w/v) NaOH. After that, the product was carried out to the next process, namely acidification, which used 72% (v/v) H₂SO₄. The resulting precipitate was then filtered and dried in an oven. Subsequently, the fiber content of the product was analyzed using the Van Soest analysis method, while the process conditions were evaluated using the BBD methodology. In this research, the highest lignin yield obtained was 17.04%, and the optimal process conditions used were 30 g mass of straw, 140°C, and 3 hours reaction time.

References

H. Noviardi, E. Masaenah, K. Indraswari, Antioxidant and Sun Protection Factor Potency of Ambon Banana White (Musa acuminata AAA) Peel Extract, Jurnal Ilmiah Farmako Bahari, vol. 11, no. 2, pp. 180–188, 2020.

K. Simanjuntak, Peran Antioksidan Flavonoid Dalam Meningkatkan Kesehatan, Bina Widya, vol. 23, no. 3, pp. 135–140, 2012.

D. N. Sari, L. A. Anggerta, A. F. Alfiana, Sunscreen from Coconut Coir Based Lignin Nanoparticles with Extraction Method and pH Shifting as an Anti-UV Material, Jurnal IPTEK Media Komunikasi Teknologi, vol. 27, no. 2, pp. 121–130, 2023.

S. Liu, T. Yu, N. Hu, R. Liu, X. Liu, High strength cellulose aerogels prepared by spatially confined synthesis of silica in bioscaffolds, Colloids Surf. A: Physicochem. Eng. Asp., vol. 439, pp. 159–166, 2013.

S. A. Lestari, L. A. Anggerta, Seleksi Proses dan Penentuan Kapasitas Pabrik Kimia Pembuatan Bahan Aktif Nanopartikel Ekstrak Lignin dari Jerami Padi, DISTILAT: Jurnal Teknologi Separasi, vol. 10, no. 4, pp. 868–879, 2024.

D. J. Horst, J. J. R. Behainne, P. P. de Andrade Júnior, J. L. Kovaleski, An experimental comparison of lignin yield from the Klason and Willstatter extraction methods, Energy for Sustainable Development, vol. 23, no. 1, pp. 78–84, 2014.

S. R. Yearla, K. Padmasree, Preparation and characterisation of lignin nanoparticles : evaluation of their potential as antioxidants and UV protectants, J. Exp. Nanosci., vol. 11, pp. 289–302, 2015.

D. Irawan, N. Muslimah, Z. Arifin, Lignin isolation from coconut coir with variation of time and concentration of NaOH in the process of alkaline delignification, Rasayan Journal of Chemistry, vol. 11, no. 4, pp. 1458–1460, 2018.

P. Kusumo, S. Biyono, Isolasi Lignin dari Serbuk Grajen Kayu Jati (Tectona Grandis) dengan Metode Klasson, Jurnal Teknik: Media Pengembangan Ilmu dan Aplikasi Teknik, vol. 19, no. 2, pp. 130–139, 2020.

Y. Variyana, R. S. C. Muchammad, M. Mahfud, Box-behnken design for the optimization using solvent-free microwave gravity extraction of garlic oil from Allium sativum L., IOP Conf. Ser.: Mater. Sci. Eng., vol. 673, pp. 012005, 2019.

M. Lubis, D. Fujianti, R. Zahara, D. Darmadi, The Optimization of the Electrocoagulation of Palm Oil Mill Effluent with a Box-Behnken Design, International Journal of Technology, vol. 10, no. 1, pp. 137–146, 2019.

M. A. Tekindal, H. Bayrak, B. Ozkaya, Y. Genc, Box-behnken experimental design in factorial experiments: The importance of bread for nutrition and health, Turkish Journal of Field Crops, vol. 17, no. 2, pp. 115–123, 2012.

D. P. Handayani, H. S. Kusuma, D. Timotius, T. Sarastika, M. Mahfud, H. Darmokoesoemo, Extraction of Centella asiatica herb using microwave-assisted solvent extraction: Optimization of crude extract using Box-Behnken design, Green Analytical Chemistry, vol. 11, pp. 100146, 2024.

L. R. Chica, C. Yamashita, N. S. S. Nunes, A. T. Negreiros, I. C. F. Moraes, A. G. Ferreira, C. R. M. Mayer, C. W. I. Haminiuk, C. C. Z. Branco, I. G. Branco, Optimizing alginate extraction using Box-Behnken design: Improving yield and antioxidant properties through ultrasound-assisted citric acid extraction, Food Chemistry Advances, vol. 5, pp. 100813, 2024.

M. S. Alam, A. Garg, F. H. Pottoo, M. K. Saifullah, A. I. Tareq, O. Manzoor, M. Mohsin, M. N. Javed, Gum ghatti mediated, one pot green synthesis of optimized Gold nanoparticles: Investigation of process-variables impact using Box-Behnken based statistical design, Int. J. Biol. Macromol., vol. 104, pp. 758–767 2017.

D. Belhaj, D. Frikha, K. Athmouni, B. Jerbi, M. B. Ahmed, Z. Bouallagui, M. Kallel, S. Maalej, J. Zhou, H. Ayadi, Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): partial characterization, in vitro antioxidant and antimicrobial activities, Int. J. Biol. Macromol., vol. 105, no. 2, pp. 1501–1510, 2017.

A. Hapid, S. Zullaikah, M. Mahfud, A. Kawigraha, M. U. Azmi, I. Haryanto, H. P. Soewoto, A. W. Go, S. Assabumrungrat, Optimization of microwave-assisted roasting: Box-behnken design for oxidation of sulfide minerals and control of atmospheric sulfur in refractory gold ore pretreatment, Case Studies in Chemical and Environmental Engineering, vol. 10, pp. 100826, 2024.

G. Amenyeku, S. J. Cobbina, W. Asare, G. K. Teye, Hydrothermal carbonization of organic waste using faecal sludge as a water source: Response surface methodology-Box Behnken design, Environmental Challenges, vol. 15, pp. 100900, 2024.

Y. L. Ni’mah, A. C. Pertiwi, S. Suprapto, Adsorption of Cu(II) on silica gel synthesized from chemical bottle glass waste: Response surface methodology-Box Behnken design optimization, S. Afr. J. Chem. Eng., vol. 48, pp. 55–62, 2024.

A. H. Jawad, I. Awad, A. S. Abdulhameed, Tuning of Fly Ash Loading into Chitosan Ethylene Glycol Diglycidyl Ether Composite for Enhanced Removal of Reactive Red 120 Dye : Optimization Using the Box – Behnken Design, J. Polym. Environ., vol. 28, pp. 2720–2733, 2020.

A. Reghioua, D. Barkat, A. H. Jawad, A. S. Abdulhameed, A. A. Al-kahtani, Z. A. Alothman, Parametric optimization by Box – Behnken design for synthesis of magnetic chitosan-benzil/ZnO/Fe3O4 nanocomposite and textile dye removal, J. Environ. Chem. Eng., vol. 9, no. 3, pp. 105166, 2021.

L. Agustini, L. Efiyanti, Pengaruh perlakuan delignifikasi terhadap hidrolisis selulosa dan produksi etanol dari limbah berlignoselulosa, Jurnal Penelitian Hasil Hutan, vol. 33, no. 1, pp. 69–80, 2015.

L. P. Johannes, T. D. Xuan, Comparative analysis of acidic and alkaline pretreatment techniques for bioethanol production from perennial grasses, Energies (Basel), vol. 17, no. 5, pp. 1048, 2024.

E. Cardona, J. Rios, J. Peña, M. Peñuela, L. Rios, King Grass: A very promising material for the production of second generation ethanol in tropical countries, Biomass Bioenergy, vol. 95, pp. 206–213, 2016.

C. Eliana, R. Jorge, P. Juan, R. Luis, Effects of the pretreatment method on enzymatic hydrolysis and ethanol fermentability of the cellulosic fraction from elephant grass, Fuel, vol. 118, pp. 41–47, 2014.

A. J. Adebayo, J. K. Ogunjobi, O. O. Oluwasina, L. Lajide, Isolation, optimization, liquefaction, and characterization of lignin from agricultural wastes, Applied Journal of Environmental Engineering Science, vol. 8, no. 4, pp. 307–328, 2022.

Y. Variyana, Y. Susanti, Optimasi Ekstraksi dari Capsicum frutescens L dengan Microwave-Assisted Soxhlet Extraction (MASE) Menggunakan Response Surface Methodology (RSM), Jurnal Riset Sains Dan Teknologi, vol. 5, no. 2, pp. 131–138, 2021.

L. A. Anggerta, F. Kurniawansyah, R. Tetrisyanda, G. Wibawa, Catalytic Synthesis of Diethyl Carbonate from Carbon Dioxide using Catalyst KI/EtONa with Propylene Oxide as Dehydration Agent and Process Optimization Based on Box-Behnken Design, International Journal of Technology, vol. 16, no. 1, pp. 243–254, 2025.